Diatom mounting adhesives - a selection
after Debes - as communicated by Raymond Hummelink.
2gms of white film gelatine
75ml acetic acid
3 gm absolute alcohol or isopropanol
1.5 gm iso-butanol
- Dissolve 2 grams of white film gelatine in 75 ml acetic acid; this can take several days. When dissolved filter. This stock solution can be stored in a refrigerator.
- Mix 3 g absolute alcohol and 1,5 g Iso-butanol* and use a pipette to add, slowly, to 5 ml of the stock solution whilst stirring. This mixture should be stored dark and cool.
- A small drop of this mixture is applied onto a clean cover glass, using a glass rod or similar. The drop should spread pretty much to the edges on its own; no manipulation is needed.
- Gelatine for photography is to be preferred over food grade (supermarket) gelatine.
- Only 0,7g of Iso-butanol can be used if preferred.
after Solliday - taken from Jim Sollidays paper
Gelatin-Acetic Acid-Ethyl Alcohol mixture
Gelatin 6 grams
Distilled Water 50 grams
Glacial Acetic Acid 50 grams
Ethyl Alcohal 8 grams
- Place the Gelatin and Distilled Water in a 200ml flask, then place the flask in a hot water bath and agitate until the Gelatin is in solution.
- When cool add the Acetic Acid and Alcohol. Filter through filter paper, discarding the first few drops that pass through the filter.
- The fixative is stored in tightly stoppered bottles, filtered occasionally.
Glacial Acetic Acid
Distilled - deionised water
- Dissolve Tragacanth in hot distilled water until thick to 1/3rd of the required final quantity.
- Add an equal quantity of absolute alcohol and then the same of Glycerine and then filter.
- Add Glacial Acetic Acid one drop at a time until it is just detectable to the nose.
after Meakin - as communicated by Raymond Hummelink.
Meakin’s fixative: (Meakin, S.H., ”The Study of Diatoms”; IV: Selected Slides):
“A small supply of fixative must be made by dissolving half an ounce of Dextrine flour in a quarter ounce of water on a water bath and adding 1½ ounces of glycerine. When dissolved, preserve the fixative by adding about 3 per cent Carbolic Acid.”.
An alternate fixative should be used!
Raymond Hummelink suggests using some glacial acetic acid and some sodium benzoate as a preservative instead of carbolic acid.
after AJ Doig - taken from Stuart R Stidolph's paper.
Egg albumen 50% and Glycerine 50%.
- Mix together by stirring fresh egg albumen with an equal amount of glycerine. Filter through the fastest paper (Whatmans No. 1); change the filter paper regularly and pour remaining deposit from one to another.
- When the mixture becomes too thick for further filtration discard and proceed again with a fresh mixture until sufficient fixative is filtered (10ml is ample). Add 1% chlorbutol and the mixture will keep indefinitely.